SymphonyDGGE Denaturing Gradient Gel Electrophoresis

Product Description

SymphonyDGGE Denaturing Gradient Gel Electrophoresis is a comprehensive system to detect DNA mutations. The system comprises a temperature control unit, stirrer, gradient mixer and casting accessories. The user-friendly vertical screw-clamp design allows rapid setup of denaturing PAGE gels. Various mutation detection techniques can be carried out with the efficient microprocessor-controlled PID temperature control unit between ambient temperature and 70°C. Among the applications of the system are heteroduplex analysis (HA), parallel denaturing gradient gel electrophoresis (DGGE) and constant denaturing gradient gel electrophoresis (CDGE). The integrated modular design provides great convenience for switching between different mutation detection techniques. The maximum 96-sample throughput enables detection of high mutation numbers within a few hours. The gradient mixer, SDGGE-GM100 is provided as standard for preparation and mixing of high and low density denaturant solutions. With the flat-base design and support handle, SDGGE-GM100 can be secured to a retort stand, providing an easy mount on a magnetic stirring plate. Not only that, the mixing chamber can accommodate a magnetic stirrer to form a linear gradient. An optional peristaltic pump is available for linear and reproducible gradient gels.

Features

  • Complete system for DNA mutation analysis
  • Maximum 96-sample throughput compatible with microplates and thermal cycler blocks
  • Four-screw vertical clamp design for rapid setup
  • Higher resolution with large format 20 x 20 cm glass plates
  • 100 ml gradient mixer with valve-controlled 50 ml reservoir and mixing chambers, able to make two 1 mm parallel denaturing gradient gels
  • Accuracy of ±0.02°C with microprocessor-controlled temperature control unit

SymphonyDGGE Applications

DescriptionDGGECGGEHA
Applicationi. Determines the denaturing conditions required to identify unknown mutations down to one base pair difference between DNA strands

ii. Increasing denaturant concentrations melt DNA in a domain-specific manner. DNA domain with lowest Tm contains the mutation or polymorphism of interest.

iii. Requires parallel DGGE – resolve DNA samples at uniform temperature in gels containing formamide and urea denaturant gradient parallel to the direction of electrophoresis

iv. The higher GC content in the sample, the harder it melts, thus progressing further into the gel before stopping.
i. Rapid screening method for multiple samples containing a known mutation

ii. DGGE is first required to determine optimal denaturing conditions for mutation identification

iii. Utilizes constant denaturant gel instead of denaturant gradient

iv. Provides higher efficiency
i. Applied for difficult detection of homodulex mutation by DGGE

ii. Denaturation and annealing of wild type and mutant DNA mixed together, typically within a PCR reaction

iii. Heteroduplexes melt at a lower denaturant concentration compared to wild type and mutant homoduplex molecules, enabling them to be identified by reduced mobility within the gel

iv. Requires parallel DGGE, or can be performed overnight in a TBE gel made from special high resolution acrylamide
SymphonyDGGE Benefitsi. SDGGE-GM100 gradient mixer and optional peristaltic pump simplify casting of denaturing gradient gels

ii. Newly designed insert and cam-caster prevent leakage during casting

iii. Microprocessor temperature control unit ensures consistent run temperature between 45-70°C

iv. High resolution 20 x 20 cm format
i. Newly designed insert and cam-caster provides leak-proof casting

ii. Optimal temperature control with microprocessor-controlled temperature unit

iii. Maximum 96-samples (48 samples per gel)
i. Newly designed insert and cam-caster provides leak-proof casting

ii. High resolution 20 x 20 cm format

iii. Gradient mixer, SDGGE-GM100 eases DGGE operation

iv. Optimal temperature control

Advanced Casting and Setup Mechanism

  • Simplifies gel casting with newly designed vertical screw-clamp technology
  • Eliminates time-consuming gel casting with just 4 screws and plate transfer
  • Built-in inner buffer chamber within PAGE insert allows setup to be completed without heavy top tanks or buffer chambers
  • Soft-silicone mat on the cam-caster base prevents leakage during gel casting

Consistent and optimal thermal control

  • Combines buffer recirculation with a heat sensor and 1.4W heating element to assist efficient temperature control within ±0.02°C
  • Allows gel temperature to be set to the melting temperature (Tm) of the amplified DNA polymorphism or mutation of interest
  • Clear and bright 4-digit 16 mm LED display for intuitive operation, 0.1°C resolution, 3 adjustable preset temperature
  • Stirred buffer recirculation for temperature stability and uniformity

Programmable power supply option

  • Melody500: 500V, 800mA, 300W outputs
  • Full versatility for different mutation detection techniques

Technical Data

W Maxi Insert and TankTemperature Control unit
Max. number of gels2 per runTemperature controlPID
Plate dimensions (W x H)20 x 20 cmOperating temperature rangeAmbient-100°C
Gel dimensions (W x H)16 x 17.5 cmWorking temperature range (DGGE)45-70°C
Spacer thicknesses0.75, 1, 1.5 and 2 mmBuffer recirculation mechanismStirring
Max. sample capacity96 samples; 48 per gelTemperature uniformity/Stability at 37°C±0.05/0.02°C
Available combs1, 5, 10, 18MC, 36MC, 48; as per W Maxi unitSetting/Display resolution0.1°C
Max. buffer volume8.5 LSafetyFluid-level float switch; isolated; IEC1010/CE
Unit dimension (W x D x H)40.5 x 17 x 44 cmStored temperature values4
Weight8 kgHeater power at 230V/ 110V AC1.4/ 1.3kW
Recommended Power SupplyGradient Mixer
Voltage500V totalTotal volume100 ml
Current800mAVolume of reservoir & mixing chambers50 ml
Power300WInternal diameter of outlet port2 mm

Resources

Documents

Data Sheet : SymphonyDGGE Denaturing Gradient Gel Electrophoresis

Selection Guide : Power Supply

Ordering Info

Cat. No.SymphonyDGGE Denaturing Gradient Gel Electrophoresis System
EB-VES-SDGGE-110VSymphonyDGGE System, 20 x 20 cm:
temperature control unit, cam-casting base, glass plates with 1 mm bonded spacers, 2 x 24-sample combs and gradient mixer, 100V
EB-VES-SDGGE-240VSymphonyDGGE System, 20 x 20 cm:
temperature control unit, cam-casting base, glass plates with 1 mm bonded spacers, 2 x 24-sample combs and gradient mixer, 240V
SDGGE-TC-115VTemperature control unit, 115V
SDGGE-TC-240VTemperature control unit, 240V
SDGGE-GM100Gradient mixer, 100 ml
SDGGE-KITSymphonyDGGE package: SDDGE, TT-DMS-18, SDGGE-PP, SDGGE-PPS16, Melody500
SDGGE-TOuter tank for SymphonyDGGE, no running module or controller
SDGGE-LLid for SymphonyDGGE
SDGGE-PPSingle channel peristaltic pump
SDGGE-PPS16Silicon tube 1.8”, 25 ft. for peristaltic pump
TT-DMS-18CycloneSpin Digital Magnetic Stirrer
Melody500-110VMelody500, 500V, 800mA, 300W, 100V
Melody500-240VMelody500, 500V, 800mA, 300W, 100V