SymphonyIEF Isoelectric Focusing

Product Description

SymphonyIEF Isoelectric Focusing is a versatile unit that covers most IEF requirements from small scale to high-throughput operation. The focusing tray can accommodate up to 12 IPG strips. It is convenient with adjustable ‘pick-and-place’ electrodes clip anywhere within the focusing tray to resolve IPG strips from 7-24 cm in length, colour-coded to prevent polarity reversal. It is also compatible with horizontal precast IEF and PAGE gels, with electrode frame that clips directly onto the cooling plate. The cooling plate built from a special grade ceramic boasts a large 26 x 26 cm surface area which enables better heat dissipation and homogenous thermal control, making it efficient for high voltage IEF applications. An optional recirculating chiller is also available which connects easily to the cooling plate via snap-lock connectors to preserve optimal operating temperatures for IPG strips (20°C) and precast gels (4°C).

The unit now comes with rehydrating tray for convenient transfer of IPG strips to the focusing tray without time-consuming removal of residual rehydration buffer. This also allows focusing tray to remain permanently in use for IEF to maximise throughput. In addition, it also provides useful storage at -20°C for focused strips before second-dimension runs.

Recommended power supply is HarmonyCS2320, 3000V 150mA, 150W. HarmonyCS3000 has 9 different program settings with 9 different parameters and voltage ramping for multi-step IEF techniques. The power supply allows setting Volt-Hours for focusing to be achieved faster at maximum voltage. You can save up to 3600 output values for data logging, collect run data via R3232 connection and it comes with free downloadable data acquisition software.

Features

  • Ideal for IPG strips and IEF gels
  • Adjustable electrodes
  • Focusing tray for maximum 12 IPG strips (7-24 cm) and rehydration tray provided
  • Large cooling platform

SymphonyIEF components:

  • Focusing Tray
  • Adjustable ‘pick-and-place’ electrodes
  • Ceramic cooling plate with snap- lock connectors
  • Rehydration tray (not shown)
  • Electrode frame for horizontal precast gels
  • Shrouded 2 mm high voltage cables (not shown)

Technical Data

IPG Strip Length

Tray Specification7 cm11 cm18 cm24 cm
Focusing tray
Electrode distance6.5 cm10.2 cm17.1 cm22.7 cm
Max. strip length25.3 cm25.3 cm25.3 cm25.3 cm
IPG strip length7 cm11 cm18 cm24 cm
Rehydration tray
Max. strip length24 cm24 cm24 cm24 cm
Recommended volume for strip rehydration3.5 ml6 ml8 ml12 ml

*IPG strips are not supplied by Hercuvan. All measurement and typical running condition are based on Serva IPG BlueStrip.

Typical Running Conditions

7 cm IPG strip
IEF step123456
Voltage (V)15030060015003000300
Time (H)0.50.50.50.50.50.5
Volt-Hours751503007507500-
18 cm IPG strip

IEF step12345
Voltage (V)30060015003000300
Time (H)0.51112<20
Volt-Hours150600150036000-

Resources

Documents

Data Sheet : SymphonyIEF Isoelectric Focusing

Ordering Info

Cat. No.SymphonyIEF Isoelectric Focusing
EB-VES-SIEFSymphonyIEF Flatbed IEF system for IPG strips and gels, with focusing and rehydration trays
EB-CL-JAZZ-115VJazz Chiller system, -20°C to 100°C, including insulated tubing and clips, 115V
EB-CL-JAZZ-240VJazz Chiller system, -20°C to 100°C, including insulated tubing and clips, 240V
EB-VES-SIEFKIT-115V1D Combination package, includes EB-VES-SIEF, EB-CL-JAZZ, Harmony2320, 115V
EB-VES-SIEFKIT-240V1D Combination package, includes EB-VES-SIEF, EB-CL-JAZZ, Harmony2320, 240V
SIEF-CPCooling Platform for IEF system
SIEF-PEReplacement positive electrode
SIEF-NEReplacement negative electrode
SIEF-EFReplacement electrode frame
SIEF-SPEReplacement spring positive electrode
SIEF-SNEReplacement spring negative electrode
SIEF-RTRehydration tray
SIEF-FTFocusing tray with adjustable electrodes
SIEF-FTPEFocusing tray with adjustable positive electrodes
SIEF-FTNEFocusing tray with adjustable negative electrodes
SIEF-TTank for SymphonyIEF
SIEF-LLid for SymphonyIEF, no cables
HarmonyCS2320-110VConsort power supply 3000V, 300mA, 300W, 110V
HarmonyCS2320-230VConsort power supply 3000V, 300mA, 300W, 230V