NS-100 NanoScan Microplate Reader
NS-100 NanoScan Microplate Reader is an excellent and versatile absorbance microplate reader that is essential for laboratories that require routine measurement of concentration or absorbance in 96-well plates. It is intended for use in various applications such as ELISA (enzyme-linked immunosorbent assays), protein quantification, cell proliferation, enzyme kinetic as well as colorimetric assays.
With fast reading speed, great performance and reproducible result, users no longer have to worry about inaccuracy or time-consuming measurements for your assays.
The NS-100 NanoScan Microplate Reader offers user-friendly interface with convenient data output, large data storage, built-in shaker for uniform cell suspension and homogenized coloured solutions. It is an economical. robust and stand-alone system that does not require any external computer. The compact design takes up only minimal space making it suitable for small laboratories.
NanoScan Intuitive Graphic Interface
Simply choose pre-set or customize your own protocols and analysis options.
Set up your 96-well plate layout by assigning the positions for positive/negative controls, standards, samples etc
After running, analyse your results with the “Curve” or “Kinetics Measurement” options
The ‘Cutoff’ value can also be set to interpret your results as ‘positive’ or ‘negative’. Print your results using a thermal printer or export via USB
A Plethora of Assay For Your Research
Custom Filters for Your Assay
Can’t find the filter for your assay? Don’t worry! You can select from a total of 28 filters in our extensive catalogue ranging from 340-750nm. Up to 8 different filters can be installed in the NS-100 for your research
Large, Intuitive Touch Screen Graphical Interface
7-inch intuitive touch screen interface allows quick navigation and set up of protocol without any PC. Simply select from any of the pre-set protocols or customize your own protocol according to your needs. Assign controls or sample type and export your data via USB or print straightaway using a thermal printer.
Fast, Accurate and Reproducible
Not only fast with a reading speed within 6 seconds,it also makes sure the results are accurate and reproducible without daunting deviations. With this reliable machine, you can save your precious time and samples and you know that the results are correct.
- 7-inch touch screen with intuitive control panel
- Can be operated as a stand-alone system or connects to printer [optional]
- 8 channel vertical optical path
- Broad wavelength range (4 standard filters: 405nm, 450nm, 492nm, 630nm with optional filters from 340-750 nm)
- Flexible data output (thermal printer or export via USB drive)
- Built-in shaker with 3 modes of speed selection
- Fast and reproducible measurement within 6 seconds
- Automatic motorized door for convenient sample loading
- Analyses include pre-processing for dual mode reads, +/- interpretation with controls, concentration determination, curve fits, and kinetic measurement analyses & curves
- Protein quantification
- Cell proliferation and growth curves
- Enzyme kinetic assay
- Colorimetric assay
|Specifications||NS-100 NanoScan Microplate Reader|
|Display||7 inch touch screen (800 x 480 pixels)|
|Plate type||96-well plates|
|Light source||Quartz-halogen lamp 6V/10W|
|Optical filter||8 channel vertical optical path. Inclusive of 4 standard optical filters: 405nm, 450nm, 492nm, 630nm|
|Half-bandwidth of filters||3-9nm|
|Dynamic range||0-4.000 Abs|
|Linearity (405nm)||0-2.000 Abs ≤±1%
2-4.000 Abs ≤±2%
|Accuracy (405nm)||±1% (0-3 Abs)
±2% (3-4 Abs)
|Precision (405nm)||CV≤0.2% (0-3 Abs)
CV≤1.0% (3-4 Abs)
|Reading speed||<6 seconds|
|Reading mode||End point, Dual Mode, Kinetic|
|Shaking mode||3 modes: slow, medium, fast|
|User interface||Integrated software or Touch Screen|
|Input Voltage||AC100-240V, 50/60Hz|
|Storage||200 programs and 100,000 records|
|Communication Ports||3 USB ports (thermal printer, mouse, USB drive) 1 ethernet port|
|Dimensions (WxDxH)||440 x 295 x 225 mm|
|Cat. No.||NS-100 NanoScan Microplate Reader|
|NS-100||NanoScan Microplate Reader NS-100 with 4 standard optical filters: 405nm, 450nm, 492nm and 630nm|
|Cat. No.||Accessories for Microplate Reader|
|NS-100-OF1||Optical filter 340nm|
|NS-100-OF2||Optical filter 380nm|
|NS-100-OF3||Optical filter 405nm|
|NS-100-OF4||Optical filter 415nm|
|NS-100-OF5||Optical filter 450nm|
|NS-100-OF6||Optical filter 492nm|
|NS-100-OF7||Optical filter 540nm|
|NS-100-OF8||Optical filter 570nm|
|NS-100-OF9||Optical filter 578nm|
|NS-100-OF10||Optical filter 590nm|
|NS-100-OF11||Optical filter 595nm|
|NS-100-OF12||Optical filter 630nm|
|NS-100-OF13||Optical filter 650nm|
|NS-100-OF14||Optical filter 690nm|
|NS-100-OF15||Optical filter 470nm|
|NS-100-OF16||Optical filter 490nm|
|NS-100-OF17||Optical filter 510nm|
|NS-100-OF18||Optical filter 520nm|
|NS-100-OF19||Optical filter 532nm|
|NS-100-OF20||Optical filter 545nm|
|NS-100-OF21||Optical filter 560nm|
|NS-100-OF22||Optical filter 562nm|
|NS-100-OF23||Optical filter 600nm|
|NS-100-OF24||Optical filter 620nm|
|NS-100-OF25||Optical filter 646nm|
|NS-100-OF26||Optical filter 663nm|
|NS-100-OF27||Optical filter 700nm|
|NS-100-OF28||Optical filter 750nm|
|NS-100-HL||Halogen Lamp for NanoScan Microplate Reader NS-100|
|NS-100-TP||Thermal Printer for NanoScan Microplate Reader NS-100|
|NS-100-PP||Printer Paper for NanoScan Microplate Reader NS-100|
- What are the common combinations of ELISA conjugate/substrate and wavelengths?
|Horseradish peroxidase (HRP)||TMB (3,3′,5,5′-tetramethylbenzidine)||450|
|ABTS (2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid)||405|
|Alkaline phosphatase (AP)||pNPP ((p-Nitrophenyl Phosphate))||405|
|BCIP (5-bromo-4-chloro-3-indoxyl phosphate)||405|
|β-galactosidase (β-gal)||ONPG ((o-Nitrophenyl-ß-D-galactopyranosidase)||420|
- What wavelength should I use for protein quantification?
- Should I use a spectrophotometer or a microplate reader for protein quantification?
Both instruments can be used. However, the limitation of a spectrophotometer is that only one sample (in a cuvette) can be measured at a time. If you have more samples and want to save time, it is advisable to use a microplate reader.
- Why are the results different for the same sample in different wells?
The quality of microplates is very important as the light source passes through the sample vertically, either from top to bottom or bottom to top. Prevent from using microplates with smudges or scratches and other forms of optical interference that may obstruct the light beam. A reference wavelength should be employed for quality control. The reference wavelength can be obtained from the manual/protocol of your specific assay.
- Why do we need to shake our samples?
Depending on the experiment, sometimes shaking is required to allow uniform cell suspension (e.g. cell proliferation assay). Shaking can also bring down the droplets stuck on the wall of the well for a more accurate measurement.
- What is the difference between end point, kinetic and dual mode?
End point mode refers to measurement of each well only once. Kinetic mode measures each well once (like end point) but repeats the process (e.g. enzyme kinetic assay, cell migration and invasion). Dual mode uses two wavelengths to measure each well (like end point) at the same time.
- Should I read my ELISA plates immediately after adding the stop solution?
It is recommended to measure the plates immediately after adding stop solution because excessive time lapse may cause the absorbance reading to shift.
- Why do I get low readings for ELISA?
This may be caused by several factors such as very low laboratory temperature (optimal working temperature is 18-25°C), incubation periods too short, reagents and buffers too cold, contaminated washer and wrong conjugates being used.
- The standard filters are not suitable for my research.
We have a total of 28 filters with various wavelengths to accommodate most applications. Kindly check the Ordering Information.
- Should we turn off the microplate reader when not in use?
Yes. We recommend turning off your microplate reader at the end of the day or when not in use for long period of time. This can extend the shelf life of tungsten halogen lamp as well as initiate a diagnostic self-check.