NS-100 NanoScan Microplate Reader

NS-100 NanoScan Microplate Reader is an excellent and versatile absorbance microplate reader that is essential for laboratories that require routine measurement of concentration or absorbance in 96-well plates. It is intended for use in various applications such as ELISA (enzyme-linked immunosorbent assays), protein quantification, cell proliferation, enzyme kinetic as well as colorimetric assays.

With fast reading speed, great performance and reproducible result, users no longer have to worry about inaccuracy or time-consuming measurements for your assays.

The NS-100 NanoScan Microplate Reader offers user-friendly interface with convenient data output, large data storage, built-in shaker for uniform cell suspension and homogenized coloured solutions. It is an economical. robust and stand-alone system that does not require any external computer. The compact design takes up only minimal space making it suitable for small laboratories.

NanoScan Intuitive Graphic Interface

Simply choose pre-set or customize your own protocols and analysis options.

Set up your 96-well plate layout by assigning the positions for positive/negative controls, standards, samples etc

After running, analyse your results with the “Curve” or “Kinetics Measurement” options

The ‘Cutoff’ value can also be set to interpret your results as ‘positive’ or ‘negative’. Print your results using a thermal printer or export via USB

A Plethora of Assay For Your Research

Custom Filters for Your Assay

Can’t find the filter for your assay? Don’t worry! You can select from a total of 28 filters in our extensive catalogue ranging from 340-750nm. Up to 8 different filters can be installed in the NS-100 for your research

Large, Intuitive Touch Screen Graphical Interface

7-inch intuitive touch screen interface allows quick navigation and set up of protocol without any PC. Simply select from any of the pre-set protocols or customize your own protocol according to your needs. Assign controls or sample type and export your data via USB or print straightaway using a thermal printer.

Fast, Accurate and Reproducible

Not only fast with a reading speed within 6 seconds,it  also makes sure the results are accurate and reproducible without daunting deviations. With this reliable machine, you can save your precious time and samples and you know that the results are correct.

Features

  • 7-inch touch screen with intuitive control panel
  • Can be operated as a stand-alone system or connects to printer [optional]
  • 8 channel vertical optical path
  • Broad wavelength range (4 standard filters: 405nm, 450nm, 492nm, 630nm with optional filters from 340-750 nm)
  • Flexible data output (thermal printer or export via USB drive)
  • Built-in shaker with 3 modes of speed selection
  • Fast and reproducible measurement within 6 seconds
  • Automatic motorized door for convenient sample loading
  • Analyses include pre-processing for dual mode reads, +/- interpretation with controls, concentration determination, curve fits, and kinetic measurement analyses & curves

Applications

  • ELISA
  • Protein quantification
  • Cell proliferation and growth curves
  • Enzyme kinetic assay
  • Colorimetric assay

Technical Data

Specifications NS-100 NanoScan Microplate Reader
Display 7 inch touch screen (800 x 480 pixels)
Plate type 96-well plates
Light source Quartz-halogen lamp 6V/10W
Wavelength range 340-750nm
Optical filter 8 channel vertical optical path. Inclusive of 4 standard optical filters: 405nm, 450nm, 492nm, 630nm
Half-bandwidth of filters 3-9nm
Dynamic range 0-4.000 Abs
Linearity (405nm) 0-2.000 Abs ≤±1%
2-4.000 Abs ≤±2%
Accuracy (405nm) ±1% (0-3 Abs)
±2% (3-4 Abs)
Precision (405nm) CV≤0.2% (0-3 Abs)
CV≤1.0% (3-4 Abs)
Resolution 0.001 Abs
Reading speed <6 seconds
Reading mode End point, Dual Mode, Kinetic
Incubator No
Shaking mode 3 modes: slow, medium, fast
User interface Integrated software or Touch Screen
Input Voltage AC100-240V, 50/60Hz
Storage 200 programs and 100,000 records
Communication Ports 3 USB ports (thermal printer, mouse, USB drive) 1 ethernet port
Dimensions (WxDxH) 440 x 295 x 225 mm
Weight 10 kg

Ordering Information

Cat. No. NS-100 NanoScan Microplate Reader
NS-100 NanoScan Microplate Reader NS-100 with 4 standard optical filters: 405nm, 450nm, 492nm and 630nm

Optional Accessories

Cat. No. Accessories for Microplate Reader
NS-100-OF1 Optical filter 340nm
NS-100-OF2 Optical filter 380nm
NS-100-OF3 Optical filter 405nm
NS-100-OF4 Optical filter 415nm
NS-100-OF5 Optical filter 450nm
NS-100-OF6 Optical filter 492nm
NS-100-OF7 Optical filter 540nm
NS-100-OF8 Optical filter 570nm
NS-100-OF9 Optical filter 578nm
NS-100-OF10 Optical filter 590nm
NS-100-OF11 Optical filter 595nm
NS-100-OF12 Optical filter 630nm
NS-100-OF13 Optical filter 650nm
NS-100-OF14 Optical filter 690nm
NS-100-OF15 Optical filter 470nm
NS-100-OF16 Optical filter 490nm
NS-100-OF17 Optical filter 510nm
NS-100-OF18 Optical filter 520nm
NS-100-OF19 Optical filter 532nm
NS-100-OF20 Optical filter 545nm
NS-100-OF21 Optical filter 560nm
NS-100-OF22 Optical filter 562nm
NS-100-OF23 Optical filter 600nm
NS-100-OF24 Optical filter 620nm
NS-100-OF25 Optical filter 646nm
NS-100-OF26 Optical filter 663nm
NS-100-OF27 Optical filter 700nm
NS-100-OF28 Optical filter 750nm
NS-100-HL Halogen Lamp for NanoScan Microplate Reader NS-100
NS-100-TP Thermal Printer for NanoScan Microplate Reader NS-100
NS-100-PP Printer Paper for NanoScan Microplate Reader NS-100

  1. What are the common combinations of ELISA conjugate/substrate and wavelengths?
Enzyme label Substrate Wavelength
Horseradish peroxidase (HRP) TMB (3,3′,5,5′-tetramethylbenzidine) 450
OPD (o-Phenylenediamine) 492
ABTS (2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid) 405
Alkaline phosphatase (AP) pNPP ((p-Nitrophenyl Phosphate)) 405
BCIP (5-bromo-4-chloro-3-indoxyl phosphate) 405
β-galactosidase (β-gal) ONPG ((o-Nitrophenyl-ß-D-galactopyranosidase) 420
Urease Urea/Bromocresol 590

  1. What wavelength should I use for protein quantification?
Protein Quantification Wavelength
Bradford 595
BCA 562
Lowry 650
Modified Lowry 750
Biuret 540

  1. Should I use a spectrophotometer or a microplate reader for protein quantification?
    Both instruments can be used. However, the limitation of a spectrophotometer is that only one sample (in a cuvette) can be measured at a time. If you have more samples and want to save time, it is advisable to use a microplate reader.
  2. Why are the results different for the same sample in different wells?
    The quality of microplates is very important as the light source passes through the sample vertically, either from top to bottom or bottom to top. Prevent from using microplates with smudges or scratches and other forms of optical interference that may obstruct the light beam. A reference wavelength should be employed for quality control. The reference wavelength can be obtained from the manual/protocol of your specific assay.
  3. Why do we need to shake our samples?
    Depending on the experiment, sometimes shaking is required to allow uniform cell suspension (e.g. cell proliferation assay). Shaking can also bring down the droplets stuck on the wall of the well for a more accurate measurement.
  4. What is the difference between end point, kinetic and dual mode?
    End point mode refers to measurement of each well only once. Kinetic mode measures each well once (like end point) but repeats the process (e.g. enzyme kinetic assay, cell migration and invasion). Dual mode uses two wavelengths to measure each well (like end point) at the same time.
  5. Should I read my ELISA plates immediately after adding the stop solution?
    It is recommended to measure the plates immediately after adding stop solution because excessive time lapse may cause the absorbance reading to shift.
  6. Why do I get low readings for ELISA?
    This may be caused by several factors such as very low laboratory temperature (optimal working temperature is 18-25°C), incubation periods too short, reagents and buffers too cold, contaminated washer and wrong conjugates being used.
  7. The standard filters are not suitable for my research.
    We have a total of 28 filters with various wavelengths to accommodate most applications. Kindly check the Ordering Information.
  8. Should we turn off the microplate reader when not in use?
    Yes. We recommend turning off your microplate reader at the end of the day or when not in use for long period of time. This can extend the shelf life of tungsten halogen lamp as well as initiate a diagnostic self-check.